BCH4053L Schedule Spring 2019

BCH4053L: Biochemistry

Laboratory Schedule Spring 2019

Week

Dates

Lecture

(Friday 1:25PM-2:15PM)

Assignments Due

Experiment

Assignments Due

M

T

W

R

1
1/7 1/8 1/9 1/10

Introduction

Chemical Properties of Amino Acids

Pre-labs 2.2 and 2.5 for AA Exps due at beginning of lecture
DAY ONE: Check-In and Lab Introduction
DAY TWO: Techniques Refresher

None

2
1/14 1/15 1/16 1/17
Protein Purification I
Pre-lab for Lysozyme Characterization 3.2 Dialysis prep Due at beginning of Lecture
DAY ONE: Properties of Amino Acids: Amino Acid Detection by Ninhydrin
DAY TWO: Properties of Amino Acids: Titration of Amino Acids

None

3
1/21 1/22 1/23 1/24
Protein Purification II
Pre-lab for Lysozyme Characterization 3.2 FPLC prep Due at beginning of Lecture
DAY ONE: No Lab
DAY TWO: Day 1 Dialysis 3.3

Report on AA Exps due by 5PM Friday 1/25/19

Bring an Egg

4
1/28 1/29 1/30 1/31
Protein Purification III
Pre-lab for Lysozyme Characterization 3.5 Concentration and Activity prep Due at beginning of Lecture
DAY ONE: Protein Extraction (FPLC) Procedure 3.3
DAY TWO: Protein Purification I (FPLC) Procedure 3.3

Writing Rubric Due by 5PM Friday 2/1/19

5
2/4 2/5 2/6 2/7
Protein Analysis
Pre-lab for Lysozyme Characterization 3.8 Day 1 and 2 MW prep Due at beginning of Lecture
DAY ONE: Protein Purification I (FPLC) Procedure 3.3
DAY TWO: Protein Quantification (Lysozyme) 3.5 A1& A2

6
2/11 2/12 2/13 2/14
Enzyme Crystallization
Pre-lab for Lysozyme Characterization 3.8 Day 3 MW prep Due at beginning of Lecture
DAY ONE: Quantification: Activity Assay 3.5 B
DAY TWO: Prepare Buffers and Gels Procedure 3.9
Report Due for Purification (Dialysis and FPLC) 5PM Friday 2/15/19

7
2/18 2/19 2/20 2/21
Enzyme Kinetics / Inhibition
Pre-lab for Lysozyme Crystallization 4.2 prep Due at beginning of Lecture
DAY ONE: Preparing Samples and Running the Gel Procedure 3.9
DAY TWO: Transfer and Western Blotting (Lysozyme)

Report Due Quantification & Activity 5PM Friday 2/22/19

8
2/25 2/26 2/27 2/28
Fundamentals of Protein Expression
Pre-lab for Kinetics 5.2 & 5.5 prep Due at beginning of Lecture
DAY ONE: Protein Crystallization I
DAY TWO: Solution Prep for Kinetics

None

9
3/4 3/5 3/6 3/7
DNA Amplification by Polymerase Chain Reaction (PCR)
Pre-lab for GFP prep 6.2 Due at beginning of Lecture
DAY ONE: Enzyme Kinetics: Activity
DAY TWO: Enzyme Kinetics: Inhibition

Lysozyme Characterization MW Due by 5PM Friday 3/8/19

Research Review Paper Due by 5PM Friday 3/8/19

10
3/11 3/12 3/13 3/14
Green Fluorescent Protein, Ligation, Transformation and Purification
Pre-lab for GFP prep Due at beginning of Lecture
DAY ONE: Prep Day
DAY TWO: PCR for GFP Plasmid

Report on Enzyme Kinetics Due by 5PM Friday 3/15/19

11
3/18 3/19 3/20 3/21 Spring Break Spring Break Spring Break Spring Break

12
3/25 3/26 3/27 3/28
DNA Fingerprinting

Pre-lab for GFP prep 6.5 Due at beginning of Lab Monday/Tuesday

Pre-lab for GFP 6.8 & 6.11 prep Due at beginning of Lecture

DAY ONE: Agarose Gel and Transformation
DAY TWO: GFP Extraction and Purification

None

13
4/1 4/2 4/3 4/4

Immunobiochemistry (ELISA)

Pre-lab for DNA prep 7.2 & 7.5 Due at beginning of Lecture
DAY ONE: GFP: GFP Purification
DAY TWO: GFP: Acrylimide Gel Analysis

Report on Protein Crystallization Due
by 5PM Friday 4/5/19

14
4/8 4/9 4/10 4/11
Final Review
Pre-lab for Elisa prep Due at beginning of Lecture
DAY ONE: Polymerase Chain Reaction: Isolation and Amplification of Template DNA
DAY TWO: DNA Fingerprinting

Report on Green Fluorescent Protein Due by 5PM Friday 4/12/19

15
4/15 4/16 4/17 4/18
Cumulative Lab Exam

DAY ONE: ELISA

DAY TWO: Check Out

Report on PCR and DNA Fingerprinting Due by 5PM 4/19/19

Research Review Paper Final Draft Due by 5PM

16
4/22 4/23 4/24 4/25
No Labs

DAY ONE: No Labs
DAY TWO: No Labs

Report on ELISA Due
by 5PM Friday 4/26/19

17
4/29 4/30 5/1 5/2
FINAL EXAM WEEK
No Labs - Have a Great Summer!

Before the Lab Meeting:

Read the lab experiment to understand its objectives, the concepts involved and the method to be used.

Highlight or underline the important concepts and key steps in the experimental procedure.

Do the Pre-Lab exercises to test your understanding of the experiment.

During the Lab Meeting

Take notes from the instructor’s introduction, to help remember key concepts and practical details.

While you work, think about what you are doing and how it contributes to the goal of the experiment.

Record data immediately and completely on the data pages to avoid losing important information.

Whenever possible do calculations and conclusions before you leave the lab, where discussion with classmates and instructor is readily available. Otherwise, do calculations and conclusions as soon as possible after the lab meeting. Ask for help if needed. Get reports completed and handed in on time.

Week	Dates				Lecture (Friday 1:25PM-2:15PM)	Assignments Due	Experiment	Assignments Due
Week	M	T	W	R	Lecture (Friday 1:25PM-2:15PM)	Assignments Due	Experiment	Assignments Due
1	1/7	1/8	1/9	1/10	Introduction Chemical Properties of Amino Acids	Pre-labs 2.2 and 2.5 for AA Exps due at beginning of lecture	DAY ONE: Check-In and Lab Introduction DAY TWO: Techniques Refresher	None
2	1/14	1/15	1/16	1/17	Protein Purification I	Pre-lab for Lysozyme Characterization 3.2 Dialysis prep Due at beginning of Lecture	DAY ONE: Properties of Amino Acids: Amino Acid Detection by Ninhydrin DAY TWO: Properties of Amino Acids: Titration of Amino Acids	None
3	1/21	1/22	1/23	1/24	Protein Purification II	Pre-lab for Lysozyme Characterization 3.2 FPLC prep Due at beginning of Lecture	DAY ONE: No Lab DAY TWO: Day 1 Dialysis 3.3	Report on AA Exps due by 5PM Friday 1/25/19 Bring an Egg
4	1/28	1/29	1/30	1/31	Protein Purification III	Pre-lab for Lysozyme Characterization 3.5 Concentration and Activity prep Due at beginning of Lecture	DAY ONE: Protein Extraction (FPLC) Procedure 3.3 DAY TWO: Protein Purification I (FPLC) Procedure 3.3	Writing Rubric Due by 5PM Friday 2/1/19
5	2/4	2/5	2/6	2/7	Protein Analysis	Pre-lab for Lysozyme Characterization 3.8 Day 1 and 2 MW prep Due at beginning of Lecture	DAY ONE: Protein Purification I (FPLC) Procedure 3.3 DAY TWO: Protein Quantification (Lysozyme) 3.5 A1& A2
6	2/11	2/12	2/13	2/14	Enzyme Crystallization	Pre-lab for Lysozyme Characterization 3.8 Day 3 MW prep Due at beginning of Lecture	DAY ONE: Quantification: Activity Assay 3.5 B DAY TWO: Prepare Buffers and Gels Procedure 3.9	Report Due for Purification (Dialysis and FPLC) 5PM Friday 2/15/19
7	2/18	2/19	2/20	2/21	Enzyme Kinetics / Inhibition	Pre-lab for Lysozyme Crystallization 4.2 prep Due at beginning of Lecture	DAY ONE: Preparing Samples and Running the Gel Procedure 3.9 DAY TWO: Transfer and Western Blotting (Lysozyme)	Report Due Quantification & Activity 5PM Friday 2/22/19
8	2/25	2/26	2/27	2/28	Fundamentals of Protein Expression	Pre-lab for Kinetics 5.2 & 5.5 prep Due at beginning of Lecture	DAY ONE: Protein Crystallization I DAY TWO: Solution Prep for Kinetics	None
9	3/4	3/5	3/6	3/7	DNA Amplification by Polymerase Chain Reaction (PCR)	Pre-lab for GFP prep 6.2 Due at beginning of Lecture	DAY ONE: Enzyme Kinetics: Activity DAY TWO: Enzyme Kinetics: Inhibition	Lysozyme Characterization MW Due by 5PM Friday 3/8/19 Research Review Paper Due by 5PM Friday 3/8/19
10	3/11	3/12	3/13	3/14	Green Fluorescent Protein, Ligation, Transformation and Purification	Pre-lab for GFP prep Due at beginning of Lecture	DAY ONE: Prep Day DAY TWO: PCR for GFP Plasmid	Report on Enzyme Kinetics Due by 5PM Friday 3/15/19
11	3/18	3/19	3/20	3/21	Spring Break	Spring Break	Spring Break	Spring Break
12	3/25	3/26	3/27	3/28	DNA Fingerprinting	Pre-lab for GFP prep 6.5 Due at beginning of Lab Monday/Tuesday Pre-lab for GFP 6.8 & 6.11 prep Due at beginning of Lecture	DAY ONE: Agarose Gel and Transformation DAY TWO: GFP Extraction and Purification	None
13	4/1	4/2	4/3	4/4	Immunobiochemistry (ELISA)	Pre-lab for DNA prep 7.2 & 7.5 Due at beginning of Lecture	DAY ONE: GFP: GFP Purification DAY TWO: GFP: Acrylimide Gel Analysis	Report on Protein Crystallization Due by 5PM Friday 4/5/19
14	4/8	4/9	4/10	4/11	Final Review	Pre-lab for Elisa prep Due at beginning of Lecture	DAY ONE: Polymerase Chain Reaction: Isolation and Amplification of Template DNA DAY TWO: DNA Fingerprinting	Report on Green Fluorescent Protein Due by 5PM Friday 4/12/19
15	4/15	4/16	4/17	4/18	Cumulative Lab Exam		DAY ONE: ELISA DAY TWO: Check Out	Report on PCR and DNA Fingerprinting Due by 5PM 4/19/19 Research Review Paper Final Draft Due by 5PM
16	4/22	4/23	4/24	4/25	No Labs		DAY ONE: No Labs DAY TWO: No Labs	Report on ELISA Due by 5PM Friday 4/26/19
17	4/29	4/30	5/1	5/2	FINAL EXAM WEEK		No Labs - Have a Great Summer!