Bridging Reaction:
Pyruvate Acetyl-CoA
Catalyzed by Pyruvate Dehydrogenase
Overall reaction:
Pyruvate + CoASH + NAD+ Acetyl-CoA + NADH + CO2
Two cosubstrate coenzymes:
Coenzyme A and NAD+
Three prosthetic group coenzymes:
thiamine pyrophosphate, lipoic acid, FAD