MASS Lab

MASS Lab Facility Details

  • Phone: (850) 644.1697
  • Location: CSL 1008 B, CSL 116

 

Mass Spectrometry Laboratory (MASS Lab) Instruments


 

JEOL AccuTOF Time-of-Flight Mass Spectrometer

Description:

Time-of-flight (TOF) mass spectrometer with reflectron geometry and conventional electrospray and nano-electrospray ionization sources. Used to determine mass/charge ratios of pure compounds and components of mixtures. Equipped with ternary gradient liquid chromatograph for LC-MS analyses of complex organic, environmental, biochemical solutions.

Specifications:

  • Model: JMS-T100LC
  • Mass Range: 6 - 10,000 m/z
  • Resolution: 6000
  • Mass Accuracy: 5 ppm RMS
  • Sensitivity: S/N > 10 for 10 pg resperine

Location: CSL 116

Company Website

 

 

Bruker Autoflex III MALDI Time-of-Flight Mass Spectrometer

Description:

Time-of-flight (TOF) mass spectrometer with matrix-assisted laser desorption/ionization (MALDI) used for identification and structural analysis of large biomolecules such as proteins, polymers, sugars, etc. The instrument has a capability of TOF/TOF technology (LIFT) for tandem MS (MS/MS), and can be utilized either linear or reflectron configuration with both positive and negative modes.

Specifications:

  • Model: AUTOFLEX III L200
  • Resolution: > 8,000
  • Mass Accuracy: < 20 ppm with extrnal calibration; < 5 ppm with internal calibration
  • Sensitivity: 500 amol [Glu1]-Fibrinopeptide (m/z 1,570) at S/N > 20

Location: CSL 1011B CSL

Company Website

 

Waters Quattro LC-Tandem Mass Spectrometer

Description:

A high performance benchtop triple quadrupole mass spectrometer designed for routine LC-MS-MS operation for structural characterization of pure compounds or components of complex mixtures. Equipped with electrospray ionization source that can be operated in either positive or negative modes. The Quattro LC-MS-MS may be coupled to a gradient HPLC system.

Specifications:

  • Model: Quattro LC
  • Inlet: Infusion pump, syringe pump, HPLC system with or without autosampler
  • Operational Modes: Daughter Ion Spectrum, Parent Ion Spectrum, Multiple Reaction Monitoring, Constant Neutral Loss Spectrum

Location: CSL 116

Company Website

 

Perkin Elmer Clarus 500 GC-MS

Description:

Perkin Elmer Clarus 500 GC-MS provides identification and structural characterization of volatile and semi-volatile organic compounds in complex mixtures. 

Specifications:

  • Mass Range: 1 - 1200 u
  • Ionization Mode: Electron Ionization
  • Electron Ionization Voltage: 10 - 100 eV
  • Mass Stability: ± 0.1 m/z over 48 Hours
  • Scan rate: Up to 6500 amu/sec

Location: CSL 116

Company Website

 

Agilent 6230 TOF-MS

Description:

Agilent 6230 TOF MS provides accurate mass analyses over a wide mass range. Ideal for organic mass spectrometry. The system can be configured to work either as LC-MS or DART-MS (for rapid detection of analytes). LC-MS configuration is available only on Tuesdays.

Specifications:

  • Sensitivity, MS Mode: 1pg: 50:1 S/N reserpine
  • Mass Resolution: 22,000@1522 m/z

  • Mass Accuracy: < 1 ppm MS Mode

  • LC-MS Mass Range: m/z 25-20,000

  • DART- MS Mass Range:  m/z 50-1200

  • Scan Speed: 30 scans/second

Location: CSL 1008 B

Company Website

 
 
Thermo Scientific iCAP RQ ICP-MS

Description:

Thermo Scientific iCAP RQ ICP-MS is capable of detecting most of the elements in the periodic table. Ideal for inorganic material analysis, geochemical exploration and pharmaceutical research.

Specifications:

  • Detection limit: < 0.5 ppt
  • Mass Range: 2 - 290 u

  • Resolution: User definable

  • Abundance Sensitivity: < 0.5 ppm @ m-1 (m= 238U)
  • Mass Stability:  ≤  ± 0.025 u per day

  • Scan Speed: > 3700 amu/s; Li to U with 40 interval masses

Location: CSL 1008 B

Company Website

 

Agilent 1260 Infinity II LC

Description:

Agilent 1260 Infinity II LC is used to separate complex chemical, biological and environmental mixtures. The system consists of a flexible pump, multisampler and variable wavelength detector. Superior quaternary mixing and a delay volume of 350 μL enable shortest run times, highest precision and accuracy.

Specifications:

  • Flow Rate: 0.001 - 5 ml/min
  • Max. Operating Pressure: 800 bar @ 5mL/min

  • Injection Range: 0.1 - 100 μL

  • Viscosity Range: 0.2 - 5 cP

  • Wavelength Detection Range: 190 - 600 nm

Location: CSL 1008 B

Company Website

 

 
 

GE ÄKTA Pure 25 M

Description:

GE ÄKTA pure 25 M chromatography system is used for reliable purification of protiens, peptides and nucleic acids.

Specifications:

  • Flow Rate: 0.001 - 25 ml/min
  • Max. Operating Pressure: 20 MPa

  • UV Monitor: 280 nm

  • Viscosity Range: 0.35 - 10 cP

Location: CSL 4501 (Cold Room)

Company Website

 

Sorvall Centrifuge

Description:

High-speed (up to 22,000 rpm) centrifuge with fixed angle and swinging bucket rotors.

Specifications:

  • Model: RC 6 PLUS
  • Max. Speed: 22,000 rpm

 

Location: CSL 116

 

 
 

Rates

Jeol Accu LC-TOF-MS : $12/hr

Bruker MALDI-TOF :  $12/hr

Waters Quattro LC-MS-MS : $12/hr

Perkin Elmer Clarus GC-MS: $12/hr

Agilent 6230 LC-TOF-MS: $20/hr

Agilent 6230 DART-TOF-MS: $20/hr

Thermo Scientific ICP-MS : $20 per sample

Agilent 1260 Infinity II LC : $3/hr

GE ÄKTA pure 25 M: $2.50/hr