Dr. Alan G. Marshall, Robert O. Lawton Distinguished Professor

Professional Preparation/Appointments

Ph.D. Stanford University (1970)
Chief Scientist, Ion Cyclotron Resonance Program, NHMFL

Contact Information

Email marshall@magnet.fsu.edu
Office B226 NHMFL 850.644.0529
Lab 1 NHMFL  

Programs of research

Analytical, Biochemistry

research specialties

Bioanalytical, Chemical Biology, Environment and Energy, Structural Biology

Research Interest

My research consists of the continuing development of new theory, techniques, and analytical/biological/environmental applications of Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. We were the first to apply Fourier transform techniques to mass spectrometry, and more than 800 FT-ICR mass spectrometry systems based on our patents and papers have been bought or built worldwide. At our National NSF High-Field FT-ICR Mass Spectrometry Facility at the National High Magnetic Field Laboratory, we currently offer 9.4, 9.4, 14.5, and 21 tesla (highest magnetic field in the world for FT/ICR) instruments. Our instruments hold world records for broadband mass resolution and mass accuracy and attract hundreds of users and collaborators from all over the world. We continue to push the FT-ICR technique to its ultimate limits for mass resolution, mass range, and sensitivity. FT-ICR MS allows us to identify 100,000 components in a mixture without prior separation (GC, LC , gels), thereby changing the whole approach to mixture analysis. We are also developing new numerical and graphical methods to recognize patterns in the enormous volume of data made available by FT-ICR MS (up to 8 Mwords per spectrum). Finally, we monitor H/D exchange by mass spectrometry to identify surface-exposed residues in proteins and protein complexes. 


Faculty Interview


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He, L.; Rockwood, A. L.; Agarwal, A. M.; Anderson, L. C.; Weisbrod, C. R.; Hendrickson, C. L.; Marshall, A. G. Diagnosis of Hemoglobinopathy and beta-Thalassemia by 21 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometry and Tandem Mass Spectrometry of Hemoglobin from Blood. Clinical Chem. 201965, 986-994. 

He, L.; Anderson, L. C.; Barnidge, D. R.; Murray, D. L.; Hendrickson, C. L.; Marshall, A. G. Analysis of Monoclonal Antibodies in Human Serum as a Model for Clinical Monoclonal Gammopathy by Use of 21 Tesla FT-ICR Top-Down and Middle-Down MS/MS. J. Am. Soc. Mass Spectrom. 2017, 28, 827-838.

Weisbrod, C. R.; Kaiser, N. K.; Syka, J. E. P.; Early, D.; Mullen, C.; Dunyach, J.-J.; English, A. M.; Anderson, L. C.; Blakney, G. T.; Shabanowitz, J.; Hendrickson C. L.; Marshall, A. G.; Hunt, D. F. Front-End Electron Transfer Dissociation Coupled to a 21 Tesla FT-ICR Mass Spectrometer for Intact Protein Sequence Analysis. J. Am. Soc. Mass Spectrom. 2017, 28, 1787-1795.

Wang, C.; He, L.; Li, D.; Brüschweiler-Li, L.; Marshall, A. G.; Brüschweiler, R. Accurate Identification of Unknown and Known Metabolic Mixture Components by Combining 3D NMR with Fourier Transform Ion Cyclotron Resonance Tandem Mass Spectrometry. J. Proteome Res. 2017, 16, 3774-3786.

Krajewski, L. C.; Rodgers, R. P.; Marshall, A. G. 126,264 Assigned Chemical Formulas from an Atmospheric Pressure Photoionization 9.4 Tesla Fourier Transform Positive Ion Cyclotron Resonance Mass Spectrum. Anal. Chem. 2017, 89, 11318-11324.